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Efficacy of Various Insecticides Against Bed Bugs. Dr. Bill Donahue Sumiko De La Vega. Nebraska Urban Pest Management Conference February 12-13, 2013. “Brood Centers”. Bed bugs are thigmotactic preferring rough surfaces such as wood or paper.
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Efficacy of Various Insecticides Against Bed Bugs Dr. Bill Donahue Sumiko De La Vega Nebraska Urban Pest Management Conference February 12-13, 2013
“Brood Centers” • Bed bugs are thigmotactic preferring rough surfaces such as wood or paper. • Cluster together in contact with each other where fecal matter, egg shells and exuvia accumulate - Semiochemicals • Return to these harborages after a blood meal. • Quiescent state while digestion takes place. • Stimulus to leave harborage? Hunger?
Repellent A chemical that makes unattractive to arthropods a habitat, food source, or oviposition site ordinarily sought and frequented. “Push-Pull” Theory – Repel and Attract - used in the same environment
Objective To evaluate the contact activity (mortality, repellency, egg distribution and hatch) of selected strains of bed bugs exposed to treated fabric substrates.
Chemicals Evaluated • Permethrin Impregnated Fabrics (3) – 0.52% a.i. • Phantom Aerosol – Chlorfenapyr 0.5% • Alpine Aerosol – Dinotefuran 0.5% • Bedlam Aerosol – Sumithrin 0.4%, MGK-264 1.6% • Bed Bug Gel - Lactic Acid 20%, Rosemary Oil 1%, Hops Flower Essential Oil 1%
Bed Bug Strains • “Harlan” – Laboratory Susceptible Strain (Ft. Dix, NJ 1973 - SRL 2008) Adults Mixed Sex Ratio • “Earl” – SRL Field Collected (Modesto, CA - 2007) Susceptible Strain – Adults Mixed Sex Ratio • “James” – SRL Field Collected (Modesto, CA - 2009) Strain, 7X Permethrin Resistant – Adults Mixed Sex Ratio
Test System * Forced Exposure non-choice test Permethrin Fabric “Choice Test” Aerosol “Choice Test”
Average percent mortality of adult bed bugs (2 Strains) confined to three types of permethrin treated fabrics (“Non-Choice”) compared with an untreated control as a continuous forced exposure lethal time (LT) evaluation
Average percent mortality of adult bed bugs (Harlan) confined to three types of permethrin treated fabrics (“Choice Test”) compared with an untreated control as a continuous exposure lethal time (LT) evaluation
Total # of Eggs, average percent hatch and average percent nymph mortality of bed bugs (Harlan) confined to three types of permethrin treated fabrics (“Choice Test”) compared with an untreated control as a continuous exposure evaluation
Mortality, distribution, average percent hatch and average percent nymph mortality of bed bugs (Harlan) confined to treated mattress ticking (“Choice Test”) compared with an untreated control as a continuous exposure evaluation
Conclusions • Reliable and predictive small scale bioassay • Sensitive – bed bug strains, chemicals (actives & formulations) • Demonstrates repellency – adults/nymphs, oviposition sites – “Push-Pull” applications, but need to understand the dynamics when including the host and harborage in human habitats • Evaluate control options based on unique bed bug behavior – “Brood Centers” - Urban IPM