Preparing and reference sources of the microbial fuel cell

1. Preparing and reference sources of the microbial fuel cell

2. Preparing Cut the carbon fibre electrode into the following measure: 0,5 cm 2 cm 3,7 cm 2,7 cm

3. Reagents Phosphate buffer (500 ml) Solve 4,08 g Disodium hydrogenphosphate (Na2HPO4) and 3,29 g Sodium dihydrogenphosphate (NaH2PO4) in 500 ml H2Odest. 1 M Glucose-solution (100 ml) Solve 18 g Glucose in 100 ml Phosphate buffer (see above) 0,01 M Methylene blue-solution (100 ml) Solve 0,32 g Methylene blue in 100 ml Phosphate buffer (see above) 0,02 M potassium hexacyanoferrate-solution (K3[Fe(CN)6]-solution, 100 ml) Solve 0,66 g potassium hexacyanoferrate in 100 ml Phosphate buffer (see above) Yeast-Suspension (30 ml) Solve 7 g dry yeast in 30 ml Phosphate buffer (see above)

4. Reference sources Microbial fuel cell NCBE (National Centre for Biotechnology Education) Science and Technology Centre The University of Reading Whiteknights, Earley Gate, Reading RG6 6BZ www.ncbe.reading.ac.uk