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Characterization and identification of bacteria

Characterization and identification of bacteria. Based on phenotype. Systematics (taxonomy) of bacteria. Study of diversity of microorganisms and their relationships Includes: clasification (ordering of B. into groups) nomenclature identification. Most important classes of characters.

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Characterization and identification of bacteria

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  1. Characterization and identification of bacteria Based on phenotype

  2. Systematics (taxonomy) of bacteria • Study of diversity of microorganisms and their relationships • Includes: • clasification (ordering of B. into groups) • nomenclature • identification

  3. Most important classes of characters • Morfological (cels including staining) • Cultural • Physiological • Nutritional • Biochemical • Ecological • Genetic • Antigenic • Phage susceptibility

  4. In classification of bacteria • As many charactes as posible should be studied • describtion of new species

  5. In identification of bacteria • Number of tests applied should be kept to a minimum • concetration for simple methods and for characters with very good distinguishing value • tests must be standardized

  6. Characteristics of primary importance I. • 1. Pure culture (make sure to have) • 2. Examine Gram-stained cells • 3. Look for motility or flagela • 4. Examine colonies

  7. Characteristics of primary importance II. • 5.Test for oxygen requirements • 6.Test for dissimilation of glucose (oxidative/fermentative) • 7.Select tests useful for distinguishing between the genera • 8.Select the test apllicable for species identification

  8. Mistakes • Determination of shape • Gram reaction • Motlity

  9. Biochemical characters • Oxidase test • Catalase test • Carbohydrate breakdown • O-F of glucose • Acid formation from sugars • Gas formation from glucose • ONPG test (beta-galactosidase activity)

  10. Biochemical characters • Reduction of nitrate to nitrite • Indole test • VP test (acetoin production) • Arginin dihydrolase test

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