1 / 9

Critical role of calnexin in Nef-mediated inactivation of ABCA1

Critical role of calnexin in Nef-mediated inactivation of ABCA1 . Lucas T. Jennelle and Michael I. Bukrinsky. Nef reduces cholesterol efflux and ABCA1 protein levels. ABCA1 Interacts with Calnexin. Calnexin Knockout Allows ABCA1 to Escape from ER. D. E. F. CNTL siRNA. CANX siRNA. G. H.

rio
Download Presentation

Critical role of calnexin in Nef-mediated inactivation of ABCA1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Critical role of calnexin in Nef-mediated inactivation of ABCA1 Lucas T. Jennelle and Michael I. Bukrinsky

  2. Nef reduces cholesterol efflux and ABCA1 protein levels

  3. ABCA1 Interacts with Calnexin

  4. Calnexin Knockout Allows ABCA1 to Escape from ER D E F CNTL siRNA CANX siRNA G H I ABCA1-GFP DsRed2-ER Co-Localization X ER

  5. Nef Inhibits Interaction between ABCA1 and Calnexin

  6. ABCA1 Co-Localizes with Calnexinand Nef Reduces Co-Localization ER D E F G Control H I J K WT Nef ABCA1-GFP DsRed2-CANX Co-Localization Overlay ER

  7. Nef Interacts With Calnexin

  8. Conclusion: Mechanism of Nef Action Final ABCA1 structure is Not properly folded and cannot mediate cholesterol efflux

  9. Acknowledgements Supported by NIH grants R01 HL093818, R21 DK072926 and R03 TW006238

More Related