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Section on formvar coated grid

Section on formvar coated grid. Hole in formvar. Stain. Osmium Fix. KMnO 4 Fix. No Osmium. Formaldehyde Fixation. Glut not washed well before OsO4. Swollen ER. Glutaradehyde precipitant. Uneven Osmium fix. Glutaraldehyde artifact. Membranous inclusions. Osmium only fixation.

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Section on formvar coated grid

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  1. Section on formvar coated grid Hole in formvar Stain

  2. Osmium Fix KMnO4 Fix

  3. No Osmium Formaldehyde Fixation

  4. Glut not washed well before OsO4 Swollen ER

  5. Glutaradehyde precipitant

  6. Uneven Osmium fix Glutaraldehyde artifact

  7. Membranous inclusions

  8. Osmium only fixation

  9. Toxic effects Poorly polymerized plastic

  10. Poor infiltration of fiber

  11. Loss of material

  12. Too thick

  13. Lead precipitant

  14. Lead

  15. Uranyl acetate crystals

  16. Holey formvar Batman!

  17. Charging in SEM

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