1 / 22

Isolation of Nucleic Acids

High MW Genomic DNA Isolation. Typical ProcedureCell Lysis0.5% SDS proteinase K (55o several hours)Phenol Extractiongentle rocking several hoursEthanol PrecipitationRNAse followed by proteinase KRepeat phenol extrac-tion and EtOH ppt. Phenol Extractionmix sample with equal volume of sat. p

risa
Download Presentation

Isolation of Nucleic Acids

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

    1. 1 Dr.Saba Abdi

    2. 2 Dr.Saba Abdi

    3. 3 Dr.Saba Abdi

    4. 4 Dr.Saba Abdi

    5. 5 Dr.Saba Abdi

    6. 6 Dr.Saba Abdi

    7. 7 Dr.Saba Abdi

    8. Using Spectroscopy to analyze DNA 8 Dr.Saba Abdi

    9. 9 Dr.Saba Abdi

    10. 10 Dr.Saba Abdi

    11. Intercalating Agents Distort the Double Helix Several hydrophobic molecules containing flat aromatic and fused heterocyclic rings can insert between the stacked base pairs of DNA. These molecules are called intercalating agents. Intercalating agents are potential Cancer-inducing reagents. 11 Dr.Saba Abdi

    12. 12 Dr.Saba Abdi

    13. 13 Dr.Saba Abdi

    14. 14 Dr.Saba Abdi

    15. DNA sequencing: the Sanger (dideoxy) method 15 Dr.Saba Abdi

    16. 16 Dr.Saba Abdi

    17. DNA sequencing: the Sanger method 17 Dr.Saba Abdi

    18. 18 Dr.Saba Abdi

    19. 19 Dr.Saba Abdi

    20. 20 Dr.Saba Abdi

    21. 21 Dr.Saba Abdi

    22. 22 Dr.Saba Abdi

More Related