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Molecular Cell Biology 5068: Foundation, Evaluation, Techniques, Investigation

This course provides a solid foundation in cell biology, teaching students to critically read and evaluate scientific literature. It also covers various techniques and methods used in the field. The course aims to develop the skills needed to define and investigate biological problems.

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Molecular Cell Biology 5068: Foundation, Evaluation, Techniques, Investigation

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  1. Molecular Cell Biology 5068 Bob Mercer, rmercer@wustl.edu 362-6924; 991-1243

  2. Goals For MCB 5068 1. Obtain a solid foundation of knowledge in cell biology 3. Be able to critically read and evaluate the scientific literature. 2. Obtain a working knowledge of available techniques. 4. Be able to define and investigate a biological problem.

  3. TA’s: Victoria Ismail rogersv@wustl.edu Aisha Lee aishalee@wustl.edu Brittany Townley townleyba@wustl.edu

  4. Molecular Cell Biology 5068 http://mcb5068.wustl.edu Lectures: Tuesday and Thursday, 8:30-10:00 AM, Erlanger Auditorium 9/18 & 10/11 in Holden Auditorium Discussion Section: Wednesday, 3-4 PM Papers assigned One-page summary/critique expected

  5. Molecular Cell Biology 5068 To Do: Visit website: www.mcb5068.wustl.edu Sign up for course Check out Self Assessment homework Visit Discussion Sections: Read “Official” Instructions

  6. Molecular Cell Biology 5068

  7. Discussion Section Techniques Protein purification using GST Fusion CRISPER/Cas 9 gene editing SDS-PAGE/Immunoblotting/Immunoprecipitation Immunofluorescence Sanger Sequencing Real time PCR Proliferation Assay/BrdU In vivo intracellular pH determination/pHluorin Size exclusion chromatography

  8. Grading: 3 in class closed book exams accounting for 75% of grade 25% of final grade based on performance in Discussion Section Percentage final grades last 2 years: 36% A 28% A- 21% B+ 14% B 0.5% B- 0.5% C+

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