Introduction

Introduction NMR spectroscopy in post-genomic era AC 7121 Spring 2005

Nobel for Magnetic Resonance • Isador I. Rabi Nobel Prize in Physics, 1944 For his resonance method for recording the magnetic properties of atomic nuclei. • Felix Blochand Edward M. Purcell, USA • Nobel Prize in Physics, 1952 • The NMR phenomenon was demonstrated for protons in 1946.

Nobel for Magnetic Resonance • Richard Ernst, Zurich, Nobel Prize in Chemistry, 1991 For his fundamental contributions to NMR methodology-Nuclear Magnetic Resonance Fourier Transform Spectroscopy http://www.nobel.se/chemistry/laureates/1991/ernst-lecture.html • Kurt Wüthrich Nobel Prize in Chemistry, 2002 NMR studies of structure and function of biological macromolecules. . http://www.nobel.se/chemistry/laureates/2002/wuthrich-lecture.html

Nobel for Magnetic Resonance • Paul C. Lauterbur(Urbana, IL) and Sir Peter Mansfield(Nottingham, UK) Nobel Prize in Physiology or Medicine, 2003 For their pioneering contributions which led to the application of magnetic resonance in medical imaging.

Nobel for Magnetic Resonance • Alexeij A. Abrikosow(Argonne, IL) and Vitalij L. Ginzburg(Moscow) Nobel Prize in Physics, 2003 For pioneering contributions to the theory of type-II superconductors, i.e., those alloys capable of withstanding the high magnetic fields that occur in MR applications.

1H NMR spectrum of ethanol • Positions • Intensities • Splitting patterns

NMR Spectrometer Varian A-60 spectrometer(1961) Bruker AV500 spectrometer(1999)

From Formula to Structure C18H21NO3 http://www.acornnmr.com/codeine/1h_chime.htm

From Sequence to function atgggaattg actacgtggt gttttacctc atacccaata tagtgggcgg cttttatatg 61 tttattatgg cgctgggggc ggccaagagg ccgagaagcc acgcctatcc cccgtggag aactacctcg tcgttgtagt tactgtgggc gatgagaggg taatgccggc tttagcggag accgtggccc agctggagag gctggggctg aggtacacag tgctctcctc ccgccccctc cccattaaaa accacatagt agtgccaaaa aagaggacg gctctaaata ccgcgcaatt ctctggttcg ttaagaatta cgccagaaac gacatgtggt atatcttcct tgacgacgac agctacccat tagatacccg atttttacgc gatattgcat attacggagc caggggatgc gtggctggca acggcgtgtt agtgccgagg cccggccgct cagccctcgc atacgcctta gactggatta ggtatttcca cgacttaact cataccgct tttccctcga ggtgctcaga aggcctatat tcggcatgca cggggaattg ttaatagtga ggggggatgt gttgaggagc atctggccgg ctatgggcga caccattacg gaggatttcc gcttcgccat ggagctctta aagcgcaggt acaagacttt tcaaacctcc acaagggtct caattaaaag ccccaactcc ctgagggact tcgtaagaca gagggccagg ggcggcgg tatatcaga ggccgccaag tataaaaacg cctattactt aatcctaacg gcctctccca tcgccctctt cctctcaacc ccggccagct ggctatacgg cttcacaata cccctcttaa tctccgcagt ctacgcctcg gtgtatatat acggtagctt aaaggcgaaa aggtatatat tagacgtgtg gctagcctca tttcttgaat taatgggctt aataatcggc ccgcgagaa aggcgaagaa cttctacgta atagacaaga ggtag

Reference Materials • Modern NMR Techniques for Chemistry ResearchAndrew E. Derome; Pergamon Books Ltd. 1987. (M) • 200 and more Basic NMR Experiments a practical Course; S. Braun, H.-O. Kalinowski, S. Berger; Wiley-VCH. (B) • Physical Chemistry4th ed.; Atkins; New York :/Freeman,/c1990. (PC) Chapter 18. • Instrumental Analysis4th ed.; Skoog and Leary; Fort Worth :/Saunders College Pub.(IA) Chapter 14. • http://www.cis.rit.edu/htbooks/nmr/ • http://web.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse • /index.htm

Introduction

Introduction

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Introduction to introduction to introduction to … Optimization

INTRODUCTION/ INTRODUCTION

Introduction

INTRODUCTION

Introduction

Introduction