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History of DNA

History of DNA. Griffith’s Experiment with Pneumonia and the accidental discovery of Transformation. Frederick Griffiths was a bacteriologist studying pneumonia He discovered two types of bacteria: Smooth colonies Rough colonies. :. When heat was applied to the deadly smooth type…

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History of DNA

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  1. History of DNA

  2. Griffith’s Experiment with Pneumonia and the accidental discovery of Transformation • Frederick Griffiths was a bacteriologist studying pneumonia • He discovered two types of bacteria: • Smooth colonies • Rough colonies :

  3. When heat was applied to the deadly smooth type… And injected into a mouse… The mouse lived! Griffith’s Experiment with Pneumonia and the accidental discovery of Transformation

  4. Griffith’s Experiment with Pneumonia and the accidental discovery of Transformation • Griffith injected the heat-killed type and the non-deadly rough type of bacteria. • The bacteria “transformed” itself from the heated non-deadly type to the deadly type.

  5. Griffith’s Experiment did not prove that DNA was responsible for transformation How would you design an experiment to prove that DNA was responsible for transformation?

  6. Avery, McCarty, and MacLeodAdded the non-deadly Rough Type of Bacteria to the Heat-Killed Smooth Type To the Heat-Killed Smooth Type, added enzymes that destroyed… Carbohydrates Lipids Proteins RNA DNA

  7. S-Type Carbohydrates Destroyed S-Type Lipids Destroyed S-Type Proteins Destroyed S-Type RNA Destroyed S-Type DNA Destroyed Conclusion: DNA was the transforming factor!

  8. The Hershey-Chase Experiment Protein coat Alfred Hershey & Martha Chase worked with a bacteriophage: A virus that invades bacteria. It consists of a DNA core and a protein coat DNA

  9. Protein coats of bacteriophages labeled with Sulfur-35 Phage • Hershey and Chase mixed the radioactively-labeled viruses with the bacteria Bacterium Phage The viruses infect the bacterial cells. Bacterium DNA of bacteriophages labeled with Phosphorus-32

  10. Protein coats of bacteriophages labeled with Sulfur-35 • Separated the viruses from the bacteria by agitating the virus-bacteria mixture in a blender DNA of bacteriophages labeled with Phosphorus-32

  11. Protein coats of bacteriophages labeled with Sulfur-35 • Centrifuged the mixture so that the bacteria would form a pellet at the bottom of the test tube • Measured the radioactivity in the pellet and in the liquid DNA of bacteriophages labeled with Phosphorus-32

  12. How does DNA replicate? Hypotheses: Conservative Semi-Conservative Dispersive

  13. Meselson-Stahl Experiment • Bacteria cultured in medium containing a heavy isotope of Nitrogen (15N)

  14. Meselson-Stahl Experiment • Bacteria transferred to a medium containing elemental Nitrogen (14N)

  15. Meselson-Stahl Experiment • DNA sample centrifuged after First replication

  16. Meselson-Stahl Experiment • DNA sample centrifuged after Second replication

  17. DNA replication E.Coli DNA polymerase I requires: 1. All four dNTPs (dATP, dGTP, dCTP and dTTP) 2. A primer chain with a free 3`-OH end 3. A template strand to which the primer is basepaired • Double-stranded DNA that is fully intact and lacking a free 3`-OH end will not be replicated (Ex: Intact circular DNA) 4. Mg2+

  18. DNA synthesis: DNA Polymerase Reaction (DNA)n + dNTP (DNA)n+1 + PPi 2Pi Primer 5` n+1→→ 3` 5` n+2 →3` Template DNA chain growth is 5’ to 3’

  19. Summary of basic mechanism of DNA replication • Replication is semiconservative • DNA polymerase requires a template-primer complex • dNTPs are the substrates for DNA synthesis • PPi breakdown to 2 Pi (catalyzed by pyrophosphatase) drives DNA synthesis • DNA Polymerase accuracy: 1 mistake every 108 bases

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