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RNA-Seq Pipeline: Gene Expression Analysis with DESeq on Galaxy Platform

Explore gene expression with RNA sequencing technology using the ELIXIR Galaxy pipeline at galaxy-ntnu.bioinfo.no. Utilize Tophat2 for alignment, FeatureCounts for counting, and DESeq for detecting differential expression. DESeq employs negative binomial distribution for estimating dispersion.

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RNA-Seq Pipeline: Gene Expression Analysis with DESeq on Galaxy Platform

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  1. RNA-seq pipeline MBV3070 18th April, 2016 Ståle Nygård Bioinformatics core facility, OUS/UiO staaln@ifi.uio.no

  2. Gene expression Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.

  3. RNA sequencing (technology)

  4. RNA sequencing (technology)

  5. ELIXIR Galaxy RNA-seq pipeline • At galaxy-ntnu.bioinfo.no • Pipeline • Tophat2 (alignment) • FeatureCounts • DESeq (differential expression)

  6. DESeqbulids on negative binomial distribution

  7. Estimating dispersion

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