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“Global” Hydrodynamic Analysis of the Molecular Flexibility of Konjac Glucomannans

This study focuses on the hydrodynamic analysis of Konjac Glucomannans (KGM), a biopolymer extracted from Amorphophallus Konjac tubers, used in health supplements and potentially in gluten removal. The research involves sedimentation velocity, size exclusion chromatography, and viscometry to analyze the molecular flexibility of KGM. Conformational analyses using different approaches suggest a semi-flexible coil conformation for KGM. The study highlights the importance of accurate characterization methods for KGM and proposes future work on KGM-gliadin complexes and KGM-insulin mixtures. 8

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“Global” Hydrodynamic Analysis of the Molecular Flexibility of Konjac Glucomannans

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  1. School of Biosciences “Global” Hydrodynamic Analysis of the Molecular Flexibility of Konjac Glucomannans Gordon Morris

  2. Outline: 1. Introduction 2.Hydrodynamic characterisations3. Conformational analyses4. Conclusions and Future Work

  3. Introduction: Why characterisation of Konjac glucomannan (KGM) is important - Widely used but poorly understood health food supplement - Reported potential interaction with wheat gliadins: application in gluten removal - Biopolymer of interest in diabetes research

  4. KGM: 1. Extracted from the tubers of Amorphophullus Konjac C. Koch 2. Water-soluble gum3.b-(14) b-D-glucose (G) and b-D-mannose (M) - G:M ratio 1:1.6 - C-6 acetylation (5 – 10 %)

  5. Hydrodynamic characterisations: Sedimentation Velocity in the Analytical Ultracentrifuge - sedimentation coefficient, s020,w - concentration dependence of sedimentation, ksSize Exclusion Chromatography coupled to Multi-Angle Laser Light Scattering - weight average molar mass, MwViscometry - intrinsic viscosity, [h]

  6. Results:

  7. Conformational analyses: 1.Sedimentation conformation zoning2.Bushin-Bohdanecky approach3. Yamakawa-Fujii approach4. Combined “global” analysis: HYDFIT5. Mark-Houwink-Kuhn-Sakurada (MHKS) relation6. Wales-van Holde & frictional ratios

  8. Sedimentation Conformation Zoning: Extra rigid rod e.g. schizophyllan Rigid rod e.g. xanthan KGM: semi-flexible coil Semi-flexible coil e.g. pectin Random coil e.g. pullulan Globular e.g. glycogen Pavlov et al. (1997). Trends in Analytical Chemistry, 16, 401-405.

  9. Bushin-Bohdanecky: • Lp ~ 8 nm • Semi-flexible coil Bohdanecky (1983). Macromolecules, 16, 1483-1493. Bushin et al., (1981). Vysokomolekulyarnye Soedineniya, A23, 2494-2503.

  10. Yamakawa-Fujii: • Lp ~ 33 nm • Rigid rod Yamakawa & Fujii (1973). Macromolecules, 6, 407-405.

  11. HYDFIT: D • Lp ~ 13 nm • ML ~ 330 g mol-1 nm-1 • Semi-flexible coil Ortega & García de la Torre (2007). Biomacromolecules, 8, 2464-2475.

  12. Summary:

  13. Conclusions1. Discrepancy between Bushin-Bohdanecky and Yamakawa-Fujii approaches - best to use non-biased HYDFIT method2. Konjac glucomannan has a semi-flexible coil conformationFuture Work1. Characterisation of KGM-gliadin complexes2. Investigation of KGM-insulin mixtures

  14. Acknowledgements: Prof. Stephen E. Harding & Ali Saber Abdelhameed, University of Nottingham, UK Dr. M. Samil Kök, University of Bolu, TurkeyDr. Jose Garcìa de la Torre & Dr. Alvaro Ortega, University of Murcia, Spain

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