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Microbial Diversity on Sinking Particles

Microbial Diversity on Sinking Particles. Goal: Why does POC concentration decrease with depth?. Does the microbial community on particles vary with depth?. T-RFLP. 16S rDNA. If yes, why?. LuxI. threshold binding. LuxR. induction. “Proteins”. “Behaviors”. Quorum Sensing.

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Microbial Diversity on Sinking Particles

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  1. Microbial Diversity on Sinking Particles Goal: Why does POC concentration decrease with depth? Does the microbial community on particles vary with depth? T-RFLP 16S rDNA If yes, why?

  2. LuxI threshold binding LuxR induction “Proteins” “Behaviors” Quorum Sensing “Talking” luxI “Listening” luxR Accumulation in the environment genes

  3. lux genes Free-living Particle associated Copies luxR/16S

  4. Q-PCR www.neb.com/nebecomm/products/dynamo_overview.asp

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