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Understanding Absorption Spectroscopy in Biophysics: Energy, Transition Probability, and Broadening

Explore the principles of absorption spectroscopy in biophysics, including energy absorption, transition probability, and broadening effects. Learn about resonance, transition dipole moments, and absorption broadening processes in electronic states. Discover the interplay of vibrational and rotational substates, Doppler shifts, and solvent effects on absorption peaks.

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Understanding Absorption Spectroscopy in Biophysics: Energy, Transition Probability, and Broadening

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  1. Absorption Spectroscopy Topic 4 part 1 Biophysics

  2. Energy and Absorption • Absorption is proportional to • Get resonance at hf = ħw = Eb – Ea www.andor.com/chemistry/?app=189

  3. Transition Probability and transition dipole moment mba = transition dipole moment = - like , it has three Cartesian coordinates

  4. Absorption Broadening • Every electronic state (eg S0 and S1) has several vibrational and rotational substates. • Each of the sharp peaks is further broadened by Doppler shifts andsolvent effects and quantum probabilities. pubs.rsc.org/ej/DT/2000/B002123G/

  5. I1 l I0 Reference Beam Splitter monochromator Detector I2 l Light Source I0 Sample with pathlength l Detector I l monochromator I0 Sample with pathlength l Detector Light Source Extinction Coefficient Or A(l) = log (I0/I) A(l) = Ce(l)l

  6. Cross Section and Connecting Macroscopic to Micropscopic • = sNA/1000

  7. Biopolymer absorption Protein Secondary Structure Protein Aromatics Blood http://life.nthu.edu.tw/~lslpc/biophys/hyperchorism.gif

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