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Mendelian Genetics. Dihybrid Crosses. I. Law of Independent Assortment. A. Dihybrid Crosses 1. move from monohybrid to dihybrid cross 2. dihybrid-investigating the inheritance of two traits at one time 3. question to be asked is a. Are two genes inherited as a package?
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Mendelian Genetics Dihybrid Crosses
I. Law of Independent Assortment • A. Dihybrid Crosses • 1. move from monohybrid to dihybrid cross • 2. dihybrid-investigating the inheritance of two traits at one time • 3. question to be asked is • a. Are two genes inherited as a package? • b. Are genes inherited independently passing on to the next generation without being affected by the first gene?
B. Traits involved • 1. Seed shape R (round) vs. r (wrinkled) • 2. Seed color Y (yellow) vs. y (green)
C. Parental generation • 1. true breeding round yellow X wrinkled green • 2. list parental genotypes • 3. list alleles carried in gametes of parental • 4. list genotype of F1 • 5. allow F1 to self-pollinate • 6. if traits are linked together would expect 3:1 ratio of round yellow : wrinkled green • 7. if traits inherited differently would expect something else
E. Explanation • 1. Analyze trait as two monohybrid crosses • 2. Total number of round : wrinkled • 3. Total number of yellow : green • 4. both come out to a 3:1 ratio • 5. evidence that the two genes are inherited independently from one another • 6. the two traits travel from one generation to the next without influencing eachother • 7. they are not tied together • 8. What does this mean at the chromosomal level?
9. Two chromosomes are traveling on different chromosomes • a. nucleus of F1 portrayed at right • b. Two possible alignments for chromosomal pairs at metaphase I • c. these alignments result in the production of four different gametes produced in equal numbers
F. Determining gametes-FOIL method • 1. meaning of foil • 2. examples of double hybrid • 3. examples using other combinations
II. Fate of Mendel • A. discouraging • B. became head of monastery-very busy and little time for experiments • C. tried to replicate results using native hawkweed • 1. very different results • 2. requires pollen to begin development • 3. male and female nucleus do not fuse • 4. zygote is a haploid being