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Summer Research Inhibition of Type I Interferon Signal Transduction Pathways by JEV infection

Summer Research Inhibition of Type I Interferon Signal Transduction Pathways by JEV infection. 中研院生醫所 R443 林宜玲老師 清大生科 05 蔡文揚 2003. 實驗室簡介. 研究 Flavivirus- 日本腦炎病毒 登革熱病毒 黃質病毒的基因體為單股正向的 RNA ，能夠作出一個大蛋白質，經過切割後形成大約 11 個病毒結構性以及非結構性蛋白質。. *. *. C. E. NS1. NS3. NS5. NS2A. NS2B. NS4A.

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Summer Research Inhibition of Type I Interferon Signal Transduction Pathways by JEV infection

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  1. Summer ResearchInhibition of Type I Interferon Signal Transduction Pathways by JEV infection 中研院生醫所 R443 林宜玲老師 清大生科 05 蔡文揚 2003

  2. 實驗室簡介 • 研究Flavivirus-日本腦炎病毒 登革熱病毒 • 黃質病毒的基因體為單股正向的RNA，能夠作出一個大蛋白質，經過切割後形成大約11個病毒結構性以及非結構性蛋白質。 * * C E NS1 NS3 NS5 NS2A NS2B NS4A NS4B prM/M : host signalase : viral protease NS2B-3 : furin or other golgi-localized protease : unknown protease

  3. Interferons (IFNs) are a family of cytokines that possess antiviral activity. Type I IFNs (viral IFNs) include IFN-a, IFN-b, IFN-w and IFN-t produced by most types of cell can produce Type II IFN (immune IFN) IFN-g produced only by NK cells, CD4+ T help cells and CD8+ cytotoxicity cells

  4. IFNs-mediated signaling pathway and anti-viral strategies

  5. Viral interference with the IFN system

  6. Our works • Construct JEdomainE/pIRES-hrGFP • Construct prMM/ME/pR21-TE12Q

  7. ATG Myc epitope pSecTagA pCR3.1 JEV-E III pCR3.1 pSecTagA BamH1 Xho1 Kpn1 Xba1 Asp7181 Hind III Apa1 Sfi1 Nhe1 Sal1 Sma1 Sal1 hrGFP pIRES Construct JEIII/pIRES-hrGFP

  8. JEV-E III/pSecTagA IRES-hrGFP Sma1(blunt end) Nhe1 T4 DNA polymerase Clean up Clean up Sal1 Sal1 Gel elution Clean up Ligase CIP Ligation Transformation

  9. Transfection • Lipofectamine (artificial membrane) • BHK-21 Immunofluorescence Assay Western plot

  10. IFA IRES-hrGFP control DAPI Myc+Texas Red JE-III/pIRES-hrGFP Myc+Texas Red DAPI

  11. Construct prMM/ME/pr21-TE12Q • Fail………….

  12. 心得 • 科學家一定要心地善良

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