MAGITTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA SLNRRIQLVE EELDRAQERL ATALQKLEEA

1. MAGITTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA SLNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRALK DEEKMELQEI QLKEAKHIAE EADRKYEEVA RKLVIIEGDL ERTEERAELA ESRCREMDEQ IRLMDQNLKC LSAAEEKYSQ KEDKYEEEIK ILTDKLKEAE TRAEFAERSV AKLEKTIDDL EVYRRKHQEL QAMQMELQSP EYKLSKLRTS TIMTDYNPNY CFAGKTSSIS DLKEVPRKNI TLIRGLGHGA FGEVYEGQVS GMPNDPSPLQ VAVKTLPEVC SEQDELDFLM EALIISKFNH QNIVRCIGVS LQSLPRFILL ELMAGGDLKS FLRETRPRPS QPSSLAMLDL LHVARDIACG CQYLEENHFI HRDIAARNCL LTCPGPGRVA KIGDFGMARD IYRASYYRKG GCAMLPVKWM PPEAFMEGIF TSKTDTWSFG VLLWEIFSLG YMPYPSKSNQ EVLEFVTSGG RMDPPKNCPG PVYRIMTQCW QHQPEDRPNF AIILERIEYC TQDPDVINTA LPIEYGPLVE EEEKVPVRPK DPEGVPPLLV SQQAKREEER SPAAPPPLPT TSSGKAAKKP TAAEVSVRVP RGPAVEGGHV NMAFSQSNPP SELHKVHGSR NKPTSLWNPT YGSWFTEKPT KKNNPIAKKE PHDRGNLGLE GSCTVPPNVA TGRLPGASLL LEPSSLTANM KEVPLFRLRH FPCGNVNYGY QQQGLPLEAA TAPGAGHYED TILKSKNSMN QPGP AREQAEAEVA SLNR IQLVE EELDR MAGITTIEAV KRK IQVLQQQ ADDAEER LEEA VA SLNRRIQLVE EE GERR ARE AV KRKIQVLQQQ ADDAEERAE ELDRAQERL ATALQKLEEA TIEAV KRKIQVLQQ 1 EKAADESERG MKVIENR ALK DEEKMELQEI QLKEAK HIAE EADRKYEEVA RK LVIIEGDL ER CREMDEQ SERG MKVIENRALK DEE AKHIAE EADRKYEE RTEERAE MDEQ DEEKMELQEI QLK EKAADESE 81 YEEEIK ILTDK TRAEFAERSV AK LEKTIDDL EVYR IRLMDQNLKC LSAAEEK HQEL QAMQMELQSP fusion site ILTDKLKEAE TRAEFAER LEKTIDDL EVYRRK C LSAAEEKYSQ K DKYEEEIK ILTDKLKE RSV AKLEKTIDDL EVYRRKHQE IRLMDQNLKC LSAAEE 161 V AKLEKTIDDL EVYRRKHQEL EYKLSKLR TSSIS DLKEVPRK LSKLRTS TIMTDYNPNY CFAGK VPRKNI TLIRGLGHGA FGE 241 LMAGGDLKS FLRE QDELDFLM E PRPS QPSSLAMLDL LHVAR 321 YLEENHFI HRDIAARNCL IGDFGMARD IYRASYYRKG GCAMLPVK GIF TSKTDTWSFG FG G GCAMLPVKWM PPEAFMEGIF TSK 401 KNCPG PVYR VLLWEIFSLG YMPYPSKSNQ E DRPNF AIILERIEYC TQDPDVINTA VLLWEIFSLG YMPYP SNQ EVLEFVTSGG RMDPPK PVYRIMTQCW QHQPED VINTA 481 LPIEYGPLVE EEE VSVRVP RGPAVE DPEGVPPLLV SQQAK REEER SPAAPPPLPT TSSGK APPPLPT TSSG VPVRPK DPEGVPPLLV SQQAK LPIEYGPLVE EEELVPV GVPPLLV SQQAKREEE 561 DRGNLGLE GSC LPGASLL PTSLWNPT YGSWFTEKPT K KNNPIAK VHGSR NKPTSLWNPT YGSWFTEKPT SNPP SELHKVHGSR NKPTSL 641 SKNSMN QPGP TAPGAGHYE LEPSSLTANM KEVPLFRLR PGAGHTED TILKSKNS VPLFRLRH FPCGNVNYGY QQQGLPLE 721 trypsin digest yields 34.4% coverage including fusion peptide LEKTIDDLE-VYR lysine-C digest yields 33.9% coverage including fusion peptide LEKTIDDLE-VYRRK glutamic-C digest yields 40.8% coverage including fusion peptideRSVAKLEKTIDDLE-VYRRKHQE proteinase K digest yields 24.5% coverage including fusion peptide VAKLEKTIDDLE-VYRRKHQEL