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Progressive 2D Gel Electrophoresis Image Transmission using Context Features & Bit-plane Method

This study at the 2004 IEEE International Conference on Networking presents a novel method involving progressive image transmission of 2D gel electrophoresis images based on context features and the bit-plane method. The approach includes the use of a JPEG progressive compression coding model and a unique transmission and reconstruction method developed by the authors. Experimental results demonstrate the effectiveness of this methodology in transmitting and reconstructing protein spot images efficiently through different phases. The conclusion highlights the advantages of the proposed approach in revealing higher-quality images with a reduced amount of transmitted data compared to conventional methods.

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Progressive 2D Gel Electrophoresis Image Transmission using Context Features & Bit-plane Method

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  1. Progressive Transmission of Two-Dimensional Gel Electrophoresis Image Based on Context Features and Bit-plane Method Source:2004 IEEE International Conference on Networking, Sensing and Control,2004, pp.1241-1246 Authors:Tung-Shou Chen, Hsien-Chu Wu, Hui-Fang Tsai, Mingli Hsieh and Shu-Fen Chiou Speaker:Shu-Fen Chiou(邱淑芬) Date:2004/12/02

  2. Outline • 2D-gel Introduction • Progressive image transmission methods • Bit-plane method • JPEG progressive compression coding model • Our Method • Experimental results • Conclusions

  3. Introduction(1/2) Two-dimensional electrophoresis gel image

  4. Introduction(2/2) Pixel value y x

  5. Progressive Image Transmission (PIT) methods • Bit-plane method (BPM). • JPEG progressive compression coding model.

  6. Bit-plane Method First phase Second phase Third phase . . .

  7. JPEG progressive compression coding model 8 DCT 8 Transmitted in zig-zag order Discrete cosine transform Divided into 8x8 blocks

  8. Our method(1/8) • Determine the Background Value • Detect Protein Spots • Transmit the image progressively • Reconstruct the image

  9. Our method(2/8) • Background Value Average pixel value= 221+190+200+…+168+155/16 =187 Background value= 221+190+200+…+231+188/7 =204 4x4 image

  10. A • 567 • 4 0 • 321 • 0 • 7 1 • 2 • 5 3 • 4 Our method(3/8) • Detect Protein Spots Chain code direction is clockwise from 0, 1, … to 7

  11. | 235 - 211| = 24 , 24 < 30 | 171 - 211| = 40 , 40 > 30 | 171 - 144| = 27 , 27 < 30 Our method(4/8) • Detect Protein Spot Background average = 211 T1 = 30 T2 = 30 Protein spot 8x8 image

  12. (1,1) + Background (211) (3,3) (6,5) Our method(5/8) • phase 1 Receiver Sender 8x8 image 8x8 image

  13. Our method(6/8) = - difference restore image original image

  14. Our method(7/8) Receiver Sender phase 2 phase 2 phase 3 phase 3 . . . . . . 8x8 image phase 10 phase 10

  15. Our method(8/8) Receiver Sender phase 11 phase 11 phase 12 phase 12 8x8 image . . . . . . . . . phase 19 phase 19

  16. Experiment results(1/8) 512x512 original 2D image

  17. Experiment results(2/8) Phase 1: Bit-plane Method JPEG model Our method

  18. Experiment results(3/8) Phase 2: Bit-plane Method JPEG model Our method

  19. Experiment results(4/8 ) Phase 3: Bit-plane Method JPEG model Our method

  20. Experiment results(5/8 ) Phase 4: Bit-plane Method JPEG model Our method

  21. Experiment results(6/8 ) Phase 5: Bit-plane Method JPEG model Our method

  22. Experiment results(7/8 ) Phase 6: Bit-plane Method JPEG model Our method

  23. Conclusions • In comparison to the other two methods: • We can reveal a better image in lesser number of phases. • The total amount transmitted is smaller.

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