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Estrus Synchronization. Estrous Cycle. GnRH FSH Estrogen LH PGF2 α. The cycle. GnRH stimulates the AP to release FSH FSH acts on the ovary to begin follicular development Follicle grows until mature and estrogen inhibits FSH This causes the AP to release LH
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Estrous Cycle • GnRH • FSH • Estrogen • LH • PGF2α
The cycle • GnRH stimulates the AP to release FSH • FSH acts on the ovary to begin follicular development • Follicle grows until mature and estrogen inhibits FSH • This causes the AP to release LH • LH acts on the follicle bursting the blister and causing ovulation
The cycle cont. • Spot on the ovary becomes the CL • CL produces progesterone maintaining pregnancy • On day 14 if the uterus does not detect and embryo then it releases PGF2α • PGF2α destroys the CL and the cycle begins again
Synchronization • Estrous synchronization gives many producers the opportunity to capture the economic benefits of artificial insemination (AI). • Because AI involves a substantial investment of labor and time, most commercial farms or ranches will not utilize this technology unless this investment can be confined to a period of less than 5 to 7 days. • To make the labor requirements of AI compatible, the estrous cycle must be synchronized so that a high percentage of treated females show a fertile, closely synchronized estrus.
Why synchronize • Because the estrous cycle is 21 days long, you can only expect to catch about 1/3 of the cycling animals in heat during the first week of the breeding period if you don’t use estrus synchronization. • Regardless of whether the animals are inseminated naturally or artificially, you can only expect 65 to 70% of them to conceive to a given insemination. • Thus, after a week of breeding to natural heats, only 21% of the eligible animals could possibly be pregnant
Why synchronize • Many estrus synchronization protocols can induce 75 to 90% of the cycling animals to display estrus within a 5 day period. • Additionally, many protocols can induce a fertile heat in as much as 50% of the anestrous cows.
Using Prostaglandins • One shot method: inject PGF2α, heat detect and breed within 7 days • Two shot method • Option 1: inject PGF2α, heat detect and breed within 7 days, inject again on day 14, heat detect and breed within 7 days • Option 2: inject PGF2α, then again on day 14, heat detect and breed
GnRH based • Give an injection of GnRH on day 0 • Begin detecting heat on day 6 • Day 7 give injection of PGF2α • On day 10 inject anything that has not shown estrus with GnRH and AI
MGA • Feed additive to control estrus • Feed at .5mg/hd/d for 14 days • Inject with GnRH 12 days after last feeding of MGA • Inject with PGF2α 7 days later • Used mainly for heifers in the feedlot
CIDR • Insert CIDR and inject with GnRH on day 0 • Day 6 inject with PGF2α • Remove CIDR on day 7 and begin heat detection • Should breed 48 hrs after removal of CIDR
Synchronization in swine • Boar exposure • Lutylase • PG 600
Boar exposure • When sows or gilts are put together and exposed to a boar everyday their cycle will synchronize with each other. • Also helps bring them into heat
Lutylase & PG 600 • Lutylase acts the same as does in cattle • PG 600 is combination of PMSG and HCG • Given in combination with lutylase will give stronger heats and better conception • We use these two with boar exposure to synchronize our sows