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Module 4 Stream Ecology Laboratory

Module 4 Stream Ecology Laboratory. Nutrients- DRAFT 5-16-2003. Measuring Nutrients in Streams. Phosphorus Ortho-Phosphorus (o-P) Total Phosphorus (TP) Nitrogen Total nitrogen (TN) Dissolved inorganic nitrogen (DIN). Nitrogen Cycle. Nutrients- orthoPhosphate. Ascorbic acid method.

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Module 4 Stream Ecology Laboratory

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  1. Module 4 Stream Ecology Laboratory Nutrients-DRAFT 5-16-2003

  2. Measuring Nutrients in Streams • Phosphorus • Ortho-Phosphorus (o-P) • Total Phosphorus (TP) • Nitrogen • Total nitrogen (TN) • Dissolved inorganic nitrogen (DIN)

  3. Nitrogen Cycle

  4. Nutrients- orthoPhosphate • Ascorbic acid method

  5. Perkin Elmer Lambda 3BUV/VIS spectrophotometer Spec 20

  6. Standards are made from a concentrated stock solution and diluted.

  7. Reagents are added to the sample, mixed then the color is allowed to develop for 20 minutes. vortexer

  8. After the blue color develops absorbance is read at 880 nm.

  9. Ammonium-N • Manual Salicylate Method

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