1 / 15

LENTIVIRAL VECTORS

LENTIVIRAL VECTORS. introduction. Lenti - latent-slow Retroviridae Enveloped , spherical viruses with a genome size of ~ 8kb Can replicate in the non dividing cells-making them one of the most efficient gene delivery vector

saddam
Download Presentation

LENTIVIRAL VECTORS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. LENTIVIRAL VECTORS

  2. introduction • Lenti- latent-slow • Retroviridae • Enveloped , spherical viruses with a genome size of ~ 8kb • Can replicate in the non dividing cells-making them one of the most efficient gene delivery vector • Although transmission is generally via infectious particles, lentiviruses are capable of infecting neighboring cells in direct contact with the host cells, without having to form extracellular particles.

  3. members • Human Immunodeficiency Virus 1 and 2 • Simian Immunodeficiency Virus • Feline Immunodeficiency Virus • Bovine immunodeficiency virus • Equine infectious anemia virus • Caprine arthritis • Caprine encephalitis virus • Puma lentivirus

  4. HOW TO MAKE A VIRAL VECTOR FROM THE VIRUS?

  5. LENTiVIRUS GENOME

  6. process

  7. The steps involved

  8. experimentally

  9. NO HELPER VIRUS- NO GOI!

  10. THE PROBLEM-GENETIC RECOMBINATION!

  11. SOLUTION

  12. Vaccines currently available

  13. SAFETY ISSUES • Lentiviruseshave a high mutation rate due to low fidelity of the lentivirusreverse transcriptase and the absence of proofreading activity. This results in the constant generation of mutants that escape from surveillance either by antibodies or specific T -cells. • Following integration of the proviral DNA into the host genome lentiviruses can remain in a quiescent state, invisible for the immune system. The initial site of infection is usually at the mucosae of the genital tracts or the intestine where the virus can replicate in the dendritic cells. • To limit viral replication in this initial stage of infection the induction of an effective mucosal immune response will be necessary. Unfortunately most vaccine strategies pursued currently do not induce mucosal immune responses. • Another problem in the immunologic control of lentivirus infection is that they destroy or interfere with the functioning of T helper cells which have a key role in the immune system.

  14. Thank you

More Related