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結構生物學期末報告

結構生物學期末報告. M93360001 洪國勝. Lac Y transform lactose with proton as “Symport”. lactose. Proton H +. Plasm membrane. cytoplasm. Lac Y. Structure Of Lac Y 1PV6. Structure Of Lac Y+TDG 1PV7. Structure analysis. 1. Substrate binding site 2.Proton interaction site

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結構生物學期末報告

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  1. 結構生物學期末報告 M93360001 洪國勝

  2. Lac Y transform lactose with proton as “Symport” lactose Proton H+ Plasm membrane cytoplasm Lac Y

  3. Structure Of Lac Y 1PV6

  4. Structure Of Lac Y+TDG 1PV7

  5. Structure analysis 1. Substrate binding site 2.Proton interaction site 3.Input/release mechanism

  6. Transmenbrane helix • There are 12 helixes transport menbrane

  7. Secondary structure • Helixes Ⅰ—Ⅵ in one site； Helixes Ⅶ—ⅩⅡ in another

  8. Hydrophilic cavity

  9. Substrate-binding site • M23、A122、E126、R144、C148、W151 in left region • D237、K358、Q359 in right region

  10. Proton translocation • Y236、D240、M299、R302、E325、H322、K319 • Almost in right region

  11. Conformation Change • Left and right region rotation 60°

  12. Sequence Homologyandstructure biofunction • Use database blast lacY sequeunce • Find the same functional residues

  13. LacY sequence

  14. Citrobacter freundii lacY

  15. Erwinia carotovora lacY

  16. Streptomyces rochei putative efflux transporter protein

  17. Sequence alignment • Use EBI database alignment this four sequence

  18. Homology remodeling

  19. Citrobacter freundii lacY

  20. Erwinia carotovora lacY

  21. Streptomyces rochei putative efflux transporter protein

  22. Result • All three species have the same substruate binding site and proton translocation site • Input/output function may be different

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