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Peptides in chemistry and biology

Peptides in chemistry and biology. Phage display Solid phase peptide synthesis and applications of synthetic libraries Native chemical ligation in protein synthesis. Scott and Smith, Science v. 249 (1990) p. 386. R 1. R 2. R 3. R 4. or. SS. R 1. R 4. R 2. R 3. Fuzeon.

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Peptides in chemistry and biology

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  1. Peptides in chemistry and biology Phage display Solid phase peptide synthesis and applications of synthetic libraries Native chemical ligation in protein synthesis

  2. Scott and Smith, Science v. 249 (1990) p. 386 R1 R2 R3 R4 or SS R1 R4 R2 R3

  3. Fuzeon

  4. Science v. 271 p. 1854 (1996)

  5. Helical Wheels - to determine amphilphilicity

  6. Native chemical ligation --Steve Kent

  7. Solid phase peptide synthesis 100-500 µ 500 nm 15 Å

  8. =

  9. Fmoc Solid Phase Peptide Synthesis

  10. Gramicidin S - a cyclic peptide antibiotic

  11. Split pool synthesis

  12. Positional scanning

  13. How to identify kinase substrates? kinase Signal output Positional scanning library

  14. Cantley et al. JBC v. 272 (1997) p. 952 PKC isoforms

  15. Wash, sequence bound peptides

  16. Results

  17. Approaches to the study of protein function Biochemistry Genetics Pharmacology http://bio.research.ucsc.edu/people/kellogg/

  18. Approaches to the study of protein function Biochemistry - requires cell-free assay

  19. 2a. Forward genetics mutagenize Screen for interesting phenotype Trace phenoptype to mutation

  20. 2b. Reverse genetics Directed deletions, point mutations Gene silencing (siRNA) Knock-out mice

  21. 3a. Forward chemical genetics Mayer et al., Science (1999) v. 286 pp. 971 - 974

  22. control + monastrol

  23. Target ID: the candidate approach http://mc11.mcri.ac.uk/wrongtrousers.html Kinesin movies http://www.proweb.org/kinesin/

  24. Reverse Chemical Genetics Kevan Shokat Curr. Op. Chem. Biol v. 5 p. 360 (2001)

  25. Ellman, et al.

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