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SSB - oligo

(A) 50. (T) 50. (C) 50. (G) 50. A. Oligo 3. B. Oligo 1. Oligo 2. 3.1. 3.1. 3.1. 3.1. 9.4. 9.4. 9.4. 9.4. 1.3. 1.3. 1.3. 1.3. 0. 0. 0. 0. 9.4. 9.4. 9.4. 3.1. 3.1. 3.1. 1.3. 1.3. 1.3. SSB ( µ M):. SSB (µ M):. 0. 0. 0. SSB- DNA. SSB-ssDNA. Free DNA. ssDNA.

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SSB - oligo

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  1. (A)50 (T)50 (C)50 (G)50 A Oligo 3 B Oligo 1 Oligo 2 3.1 3.1 3.1 3.1 9.4 9.4 9.4 9.4 1.3 1.3 1.3 1.3 0 0 0 0 9.4 9.4 9.4 3.1 3.1 3.1 1.3 1.3 1.3 SSB (µM): SSB (µM): 0 0 0 SSB- DNA SSB-ssDNA Free DNA ssDNA 9 8 1 0 1 2 5 6 7 4 3 3 1 2 4 5 6 8 9 7 1 1 1 3 2 1 1 1 1 1 1 1 1 0 6 5 1 4 3 2 Excess cold 50-nt oligo Excess cold 20-nt oligo 15 nt 30 nt 40 nt 50 nt 20 nt C D 50 50 100 100 No SSB No SSB 3.1 3.1 3.1 3.1 3.1 9.4 9.4 9.4 9.4 9.4 10 20 10 20 1.3 1.3 1.3 1.3 1.3 SSB (µM): 0 0 0 0 0 0 0 5 SSB- DNA SSB - oligo Free DNA 50-nt oligo 20-nt oligo 5 3 1 2 4 6 7 8 9 10 1 1 1 1 1 1 1 1 1 1 2 7 8 6 3 4 5 9 2 0 5 6 8 9 10 11 12 13 3 4 7 2 1

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