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Let’s Clone a Mouse

Let’s Clone a Mouse. Somatic Cell Nuclear Transfer. Remember what you learned yesterday?. Artificial Embryonic Twinning- when a scientist mimics natural cloning by separating an embryo and allowing it to briefly develop in a P etri dish before implanting into a surrogate

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Let’s Clone a Mouse

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  1. Let’s Clone a Mouse Somatic Cell Nuclear Transfer

  2. Remember what you learned yesterday? Artificial Embryonic Twinning- when a scientist mimics natural cloning by separating an embryo and allowing it to briefly develop in a Petri dish before implanting into a surrogate Somatic Cell Nuclear Transfer- when the nucleus of a somatic cell is transferred into an enucleated egg (egg where nucleus is removed) and is then placed in a surrogate

  3. Ok, so let’s begin… On your lab bench you have 4 Petri dishes, 3 mice, 1 mouse pup, and a morula (a solid ball of cells resulting from division of a fertilized ovum).

  4. You also have questions. Try to answer these as you go. Better yet, see how many you can answer now! It’s a good way to see how much you remember from yesterday’s class discussion.

  5. Color the cells. Color the cells as follows: Cumulus Cell Nucleus and Cumulus Cell = Blue Egg Cell Nucleus and Egg Cell = Blue Morula (ball of cells) = Green

  6. Color the mice. Color the 3 large female mice as follows: Somatic Cell Donor = Brown Egg Cell Donor = Black Surrogate Mother = White Don’t color the mouse pup yet!

  7. Somatic Cell Donor From the brown mouse, cut out the Cumulus Cell and place it in Petri Dish #1. Remember, this is the mouse you are cloning!

  8. Egg Cell Donor From the black mouse, cut out the Egg Cell and place it in Petri Dish #2. THINK: Do you need this mouse’s DNA?

  9. Enucleate the Egg Cell Cut out and discard the nucleus from the Egg Cell in Petri Dish #2. Move the enucleated Egg Cell to Petri Dish #3. THINK: At this point, is there any DNA in the egg?

  10. Cumulus Cell Cut out the nucleus from the Cumulus Cell in Petri Dish #1.

  11. Transferring… Transfer the Cumulus Cell Nucleus into the Enucleated Egg Cell in Petri Dish #3 by taping them together on the back. THINK: An adult mouse has 40 chromosomes. How many chromosomes does the egg cell have right now?

  12. Waiting time! Transfer the Egg Cell with the newly replaced nucleus onto Petri Dish #4 and let it rest for a minute. This waiting time represents the 1-6 HOURS that the new nucleus needs to adjust to the Egg Cell.

  13. Now it’s a morula! After it is chemically stimulated, the new Egg Cell divides into a ball of cells called a Morula. Cover the new Egg Cell with the Morula.

  14. Surrogate After the Egg Cell divides into a Morula, it is placed into the womb of the Surrogate Mother. After about 19 days, the Surrogate Mother will give birth to a new Mouse Pup.

  15. THINK: Which adult mouse will the mouse pup resemble? What color will it be? Color the newly delivered Mouse Pup this color.

  16. Tidy your lab station- throw away your trash paper bits and place mouse and keyboard up.Finish answering questions.

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