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Koch ’ Postulates. 병원균과 질병의 지속적 연관성 분리 , 순수배양 건강한 개체에 접종시 동일한 증상 재분리 후 동일한 병원균 확인. Plant vs. Animal. No antibody, T cell response No circulating cell Systemic signal through plant Disposable and replaceable parts in plant. Gene for Gene theory. Avr : avr (avirulence) gene
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Koch’ Postulates • 병원균과 질병의 지속적 연관성 • 분리, 순수배양 • 건강한 개체에 접종시 동일한 증상 • 재분리 후 동일한 병원균 확인
Plant vs. Animal • No antibody, T cell response • No circulating cell • Systemic signal through plant • Disposable and replaceable parts in plant
Gene for Gene theory • Avr : avr (avirulence) gene • R : r (resistance) gene • R * Avr = Resistance • R * avr = Susceptible • r * Avr = Susceptible • R * avr = Susceptible
Signal Molecules • ROD, reactive oxygen species • NO, nitric oxide • SA, salicylic acid • JA, jasmoic acid • C2H2, ethylene
신호전달 후 • HR, hypersensive response • Phytoalexins • PR, pathogenesis related proteins • Cell wall reinforcing, H2O2 • Ca++ • Active oxygens • SA
R and Avr interactions • PAMP(pathogen associated molecular pattern) through TTSS(type III secretion system), Fls2(flagellin receptor) • AvrRpm1 increases phosphorylation of Rin4 and decrease PAMP signaling • AvrRpt2, protease cleaves Rin4 • Rpm1 interact with Rin4 and detect change in Rin4
내병성 육종 Exogeneous genes - pathogen origin (M, Coat proteins, replicase) - plant origin (RIP) Endogenous genes - SAR - HR - PR - Signal transduction
내병성 분자육종 • Engineering of R for broad spectrum • Expression of R in an inducible manner - broad specificity of pathogens - multifactorial defense mechanism - only w/I infected tissue • 내병성 육종의 한계: 병원균의 진화