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Do Now: 3/16

Do Now: 3/16. Period 4: Science & Computers 3 rd period today – get right to work! Period 2: Radiation levels in Tokyo are 20 times above background levels, with recent measurements about 10 μ Sv /day. Remember the 1% / 5 year cancer threshold we talked about yesterday is 1 Sv .

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Do Now: 3/16

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  1. Do Now: 3/16 Period 4: Science & Computers 3rd period today – get right to work! Period 2: Radiation levels in Tokyo are 20 times above background levels, with recent measurements about 10 μSv/day. Remember the 1% / 5 year cancer threshold we talked about yesterday is 1 Sv. 1. The Tokyo Metropolitan Government has assured residents of Japan’s capital city that the area is still safe. Do you agree? Justify your answer. 2. 100 mSv/hr is considered to be the level “dangerous to human health.” How many hours of exposure at this level will produce the 1% cancer effect?

  2. Syllabus Update • There is NO CHANGE to the assigned readings. • GEL ELECTROPHORESIS is now today’s topic, because of our “breaking science news” day yesterday. • GENOMICS / BIOINFORMATICS will no longer be a topic in class. • There is NO CHANGE to the assigned readings.

  3. GEL ELECTROPHORESIS • WHAT IT IS: A method for separating DNA fragments based on their size. • WHY IT’S USEFULL: • Isolating important sequences from others • Measuring & mapping DNA sequences • Identification (DNA fingerprinting) • HOW IT WORKS: Different sized DNA molecules move through a gel at different speeds, causing them to separate.

  4. Virtual Lab • http://learn.genetics.utah.edu/content/labs/gel/

  5. Short fragments of DNA move faster through gel

  6. Gel Electrophoresis in Criminal Investigations • Suspect & crime scene DNA collected. • PCR with standard primers • Cut samples with same restriction enzyme • Separate DNA fragments by gel electrophoresis.

  7. DNA Paternity Testing • Each fragment seen in child sample must come from mother or father. • Which gel has the right father?

  8. Plasmid Mapping Extra Credit • You have been given gel electrophoresis data from the digestion of a fictitious plasmid, pOLY1, with several restriction enzymes. • Each enzyme cuts the plasmid in one or two locations. • Use the fragment sizes to make a map that shows how the restriction sites are arranged on the plasmid. Indicate the distance between sites in kbp.

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