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Acc206 ( –––). Acc130 (+ –– ). Acc201 ( –––). Acc108 (++ – ). Acc719 (+ –– ). AccUP1 ( –––). AccUP3 ( –––). Acc729 (+ ––). Acc224 ( –––). Acc709 (+ ––). Nipponbare ( –––). Acc710 (+++). Acc220 ( –––). Tsuyuake Pikm (+++). Acc724 ( –––). Acc712 ( –––).
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Acc206 (–––) Acc130 (+––) Acc201 (–––) Acc108 (++–) Acc719 (+––) AccUP1 (–––) AccUP3 (–––) Acc729 (+––) Acc224 (–––) Acc709 (+––) Nipponbare (–––) Acc710 (+++) Acc220 (–––) Tsuyuake Pikm (+++) Acc724 (–––) Acc712 (–––) Acc504 (+––) Acc435 (–––) Acc563 (++–) Acc415 (–––) Acc848 (++–) Acc417 (–––) Acc757 (++–) Acc718 (–––) Kanto 51 Pik (+++) Acc440 (–––) Kusabue Pik (+++) Acc868 (–––) K60 Pikp (+++) Acc1 (–––) Acc414 (++–) Acc624 (–––) Acc101 (–––) Acc420 (+––) Acc715 (–––) Acc761 (+––) Acc706 (–––) Acc706 (–––) Acc709 (+––) Acc624 (–––) Acc710 (+++) Acc715 (–––) Acc729 (+––) Acc724 (–––) Acc719 (+––) Acc440 (–––) Acc130 (+––) Acc108 (++–) Acc868 (–––) Acc420 (+––) Acc101 (–––) Acc761 (+––) Acc718 (–––) Acc414 (++–) Acc1 (–––) K60 Pikp (+++) Acc201 (–––) Acc504 (+––) Acc206 (–––) Acc563 (++–) AccUP1 (–––) Acc757 (++–) AccUP3 (–––) Acc848 (++–) Tsuyuake Pikm (+++) Acc220 (–––) Kusabue Pik (+++) Nipponbare (–––) Kanto 51 Pik (+++) Acc224 (–––) 0.01 0.02 Ashikawa et al. Supplementary Fig. S3 a b Clade 1 Clade 2 Clade 1 Clade 2 First gene Second gene Fig. S3. Phylogenetic trees of alleles at the rice blast resistance Pik locus. a First gene; b second gene. The trees were generated from nucleotide sequences of the entire coding regions by MEGA v. 4.0 software. The disease reaction of each line to blast isolate Ina86-137 is in parentheses: +++ indicates resistance (no lesions or no specks), ++– indicates partial resistance (some brown specks), +–– indicates partial resistance (some lesions with brown margins), ––– indicates susceptible (developed lesions).