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Quantification of actin bundling by HsACTN4 and SpAin1. Example impages Yellow : 1 filament Red : 2 Green : 3 Purple : 4 Blue : 5. +100 nM HsACTN4. 3 uM actin. +10 nM HsACTN4. +30 nM HsACTN4. +100 nM SpAin1. +30 nM SpAin1. +10 nM SpAin1. Blue: HsACTN4 Orange: SpAin1.
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Example impages Yellow: 1 filament Red: 2 Green: 3 Purple: 4 Blue: 5 +100 nM HsACTN4 3 uM actin +10 nM HsACTN4 +30 nM HsACTN4 +100 nM SpAin1 +30 nM SpAin1 +10 nM SpAin1
+30 nM HsACTN4 +100 nM HsACTN4 +10 nM HsACTN4 3 uM actin +100 nM SpAin1 +10 nM SpAin1 +30 nM SpAin1
+30 nM HsACTN4 +100 nM HsACTN4 +10 nM HsACTN4 3 uM actin +100 nM SpAin1 +10 nM SpAin1 +30 nM SpAin1