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Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877

Assembly of a random array. Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877. 96 well microtiter plate- 1 fibers bundle per well 1 bundle ~50,000 glass optical fibers w/ silica bead attached 1,536 bead types exist: yields @ 30-fold redundancy per SNP. GoldenGate SNP Genotyping Assay.

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Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877

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  1. Assembly of a random array Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 • 96 well microtiter plate- 1 fibers bundle per well • 1 bundle ~50,000 glass optical fibers w/ silica bead attached • 1,536 bead types exist: yields @ 30-fold redundancy per SNP

  2. GoldenGate SNP Genotyping Assay J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004

  3. Genotyping data from one bundle J-B Fan et al. Cold Spring Harbor Symposia on Quantitative Biology, LXVIII, 69-78, January 2004

  4. Decoding images from eight stages Kevin L. Gunderson et al. Genome Res. 2004; 14: 870-877 • Stage-by-Stage • Core-by-Core

  5. Genotype Calling – More difficult SNP

  6. Mean GenCalls Across SNPs

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