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Genetic Engineering: Utilizing EcoRI and XbaI Digest to Probe Marker Genes

This study explores the application of EcoRI and XbaI digest in genetic engineering, focusing on marker genes Ubi1:CBF3 and ABF3. The analysis involves DNA manipulation techniques to investigate gene expression. The study delves into the use of MAR probes and EcoRV digest, providing insights into genetic modifications in plants. NotI digest is employed for supplemental analysis, enhancing understanding of gene manipulation methodologies. The research highlights the significance of EcoRI and XbaI digest in probing gene markers, offering valuable insights into genetic engineering approaches. 8 Relevant

salvatore-phelan
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Genetic Engineering: Utilizing EcoRI and XbaI Digest to Probe Marker Genes

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  1. A Ubi1:CBF3 RI X X RI RI BR BL Ubi1 CBF3 3’PinII 35S Bar 3’nos MAR MAR EcoRI digest 1.3 kb MAR probe MAR probe XbaI digest CBF3 probe Ubi1:ABF3 RI RI RI RV N BR BL Ubi1 ABF3 3’PinII 35S Bar 3’nos MAR MAR EcoRI digest MAR probe 3.4 kb MAR probe EcoRV + NotI digest ABF3 probe Supplemental Figure 1A

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