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A. Ubi1:CBF3. RI. X. X. RI. RI. BR. BL. Ubi1. CBF3. 3’PinII. 35S. Bar. 3’nos. MAR. MAR. Eco RI digest. 1.3 kb. MAR probe. MAR probe. Xba I digest. CBF3 probe. Ubi1:ABF3. RI. RI. RI RV. N. BR. BL. Ubi1. ABF3. 3’PinII. 35S. Bar. 3’nos. MAR. MAR.
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A Ubi1:CBF3 RI X X RI RI BR BL Ubi1 CBF3 3’PinII 35S Bar 3’nos MAR MAR EcoRI digest 1.3 kb MAR probe MAR probe XbaI digest CBF3 probe Ubi1:ABF3 RI RI RI RV N BR BL Ubi1 ABF3 3’PinII 35S Bar 3’nos MAR MAR EcoRI digest MAR probe 3.4 kb MAR probe EcoRV + NotI digest ABF3 probe Supplemental Figure 1A