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EGFP. Figure S3: DNA methylation analysis on SFFV and EF1 α promoters in P19 cells following the lentivirus transduction. SFFV. SP1. Enhancer core. CAAT. TATA. 23 CpGs. A. B. EGFP. EF1a. CAAT. TATA. 21 CpGs. C.
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EGFP Figure S3: DNA methylation analysis on SFFV and EF1α promoters in P19 cells following the lentivirus transduction. SFFV SP1 Enhancer core CAAT TATA 23 CpGs A B EGFP EF1a CAAT TATA 21 CpGs C A: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a, at day 4 post-transduction, showing a 45 to 65% methylated CpGs from 6 of 12 (50%) analysed vector copies. B: Methylation analysis on SFFV in P19 cells with 0.41 VCN shown in Figure S2a,at day 14 post-transduction, showing a 65 to 100% methylated CpGs from 7 of 12 (58%) analysed vector copies. C: Methylation on EF1α in P19 cells with 0.38 VCN shown in Figure S2b, at day 14 post-transduction, showing a 40 to 100% methylated CpGs from 5 of 11(45%) analysed vector copies. Solid boxes. methylated CpGs