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Implementation of Subspecialty Whole Slide Image Teaching Sets for Residency Education. Bryan Dangott , M.D. University of Utah Hematopathology Fellow. The UPMC Health System. Diversified environment ( academic hospitals, community hospitals) 19 hospitals with another 18 affiliations
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Implementation of Subspecialty Whole Slide Image Teaching Sets for Residency Education Bryan Dangott, M.D. University of Utah Hematopathology Fellow
The UPMC Health System • Diversified environment ( academic hospitals, community hospitals) • 19 hospitals with another 18 affiliations • 2 million + outpatient visits per year
University of Pittsburgh Medical Centerwww.upmc.com 29 Western PA Counties 19 Hospitals 40 Cancer Center Locations
Pathology Centers of Excellence (COEs) at UPMC UPMC Presbyterian Dermatopathology ENT/Thyroid Pathology Gastrointestinal Pathology Hematopathology Neuropathology Thoracic/Mediastinal Pathology Transplant Pathology UPMC Shadyside Pathology Informatics Genitourinary Pathology Bone/Soft Tissue/Melanoma Pathology UPMC Magee-Womens Hospital Breast Pathology Gynecologic Cytopathology Obstetric/Gynecologic Pathology UPMC Childrens Hospital Pediatric Pathology
Background • UPMC is a multi-site institution • Residents rotate at different facilities • Problems with glass sets • Only available to one person at any given time • Missing slides • Broken • Disorganized • Degradation (cover slip adhesion, stain quality)
Digital Teaching Material • AP Teaching Site (http://path.upmc.edu/cases/index.html) • 700 cases • Prepared by the residents • Gross images, microscopic snap shots • Comprehensive discussions
Creating Whole Slide Image Sets • Scanning began in 2005 with GU • Over 2900 whole slide images • General Unknowns collection • Genitourinary • Head and Neck • Thoracic • Hematopathology • Under Development • GI, Bone and Soft tissue, Cytology
Infrastructure • Storage space • Storage Area Network for image files • 20 terabytes. • Supplemental file storage space. • Web hosting space.
Servers • Image server • Aperio ImageServer • Webscope, Flash viewer • ImageScope, Desktop Application • Zeiss, Java viewer • Web servers • Apache • IIS • ColdFusion
Database Backend • Oracle • Unknowns • GU • Thoracic • Head and Neck • Microsoft Access • Hemepath • Testing Interface
Front End Language • ColdFusion • Database searching • Page presentation and formatting • Javascript • Used to selectively display portions of the page • Selects the viewer to launch based on link
Basic Procedures • Gather data • Clinical Information • Diagnostic Material • Slides • Radiology • Gross Images • Flow, Cytogenetics, Molecular data • De-identify data
Scan Hardware • Aperio Scanscope XT (120 slide loader) • Aperio Scanscope CS (5 slide loader) • Zeiss Mirax MIDI (12 slide loader) • Hamamatsu (210 slide loader)
Scanning Challenges • Tape style cover slips • Scratched / damaged slides • Uneven tissue sections • Very thin sections of fatty tissue. • Peripheral smears / Aspirate smears • Cytology specimens
Scanning Challenges • Cytology and hematopathology • Focus points • Depth of field • Scan time • 40x hemepath • 30 bone marrow aspirate slides (~24 hrs) • Batch scanning
Personnel • Web Developer – Thomas Harper • Slide Scanning – Jon Duboy • Server Administration – Ishtiaque Ahmed • Project Coordinator – Doris Dayoub • Project Oversight – Anil Parwani, MD, PhD
Challenges • Managing images, web page code, clinical data sources. • Customization of individual sites • Each of the subspecialties has different needs
From Template to Flexible • Hemepath vision • More slides • Higher magnification (40x minimum) • More slide area (smears) • More data types
Two Projects at a Time • Hemepath teaching set was built simultaneously with a testing interface. • Required architecture flexibility • Required more involvement with the developers
Hemepath Data Types • CBC • Differential counts • Molecular data • Immunophenotyping • Flow Cytometry • Cytogenetics
Hemepath Database Design • Access • Up front design required a lot of interaction • Hemepath wanted to manage the database • This eliminated building a web interface for data entry • Allowed additional development to enhance data entry
Work Flow Improvements • Batch data download from APLIS • Tab delimited text file • Batch data processing • Vbscript • Notepad++ vs Excel • Data Entry • Copy and paste data fields • Link integration
Hemepath File Naming • File naming conventions • CASE# - TYPEID - FREETEXT • WSI • 001-CBX-HE • Supplemental Files • 001-FLO-CD2,CD3,CD4,CD8