Today’s Plan: 9/16/09

1. Today’s Plan: 9/16/09 • Bellwork: • Watch Video clip (10 mins) • Finish Mutations and Operons (15 mins) • Read/Pair/ Share about diabetes articles (15 mins) Read 1 insulin article. Your seat partner will do the other. Compare info and do journal • Why do diabetics need insulin? • What does insulin do? • What sources of insulin are available to diabetics? • What are the risks of using animal-derived insulin? • Notes on Recombinant DNA and restriction enzymes (20 mins) • Agriculture article/questions (the rest of the period) • Pack/Wrap-up (last few mins of class)

2. Today’s Plan: 9/17/09 • Bellwork: Ag applications article and questions (20 mins) • Notes on RFLP and other technology (30 mins) • RFLP Activity (20 mins) • The rest of the period: • Gene Therapy Article and questions • Pack/Wrap-up (last few minsof class)

3. Today’s Plan: 9/18/09 • Bellwork: Q&A (10 mins) • Vocab quiz and Unit 2 Test (as needed) • If you finish early, work on missing tasks • Pack/Wrap-up (last few mins)

4. Today’s Plan: 9/21/09 • Bellwork: Blue Diamond #5 (20 mins) • Portfolio Update (10 mins) • Go over Friday’s test (35 mins) • Finish test corrections and missing work (the rest of class) • Pack/Wrap-up (last few mins of class)

5. Today’s Plan: 9/24/08 • Bellwork: Re-cap on crime scene (10 mins) • Crime Scene analysis (the rest of the period) • Pack/Wrap-up (last few mins)

6. Today’

7. Recombinant DNA • DNA can be manipulated to contain certain genes. Manipulations are performed by cutting DNA, inserting what we want, and inserting the DNA back into a cell. • A manipulated piece of DNA, that contains “spliced” pieces is called recombinant DNA • The recombinant DNA comes from cloning vectors-carrier DNA molecules that can be cut and spliced with new genes • Most cloning vectors in biotechnology are bacterial plasmids

8. Transplanting Genes • 1st –cloning vector is removed from bacterial cells • 2nd-restriction enzymes that recognize specific base sequences on the DNA cut the plasmid at pre-determined sites, creating sticky ends • 3rd-the donor gene is inserted, closing the plasmid back into its ring shape • 4th-the new, recombinant DNA is inserted back into the bacterium. When the bacteria goes through binary fission, the donor gene is cloned too. The bacteria is now called a transgenic organism

9. Checking for Donor Gene Uptake • As you know, not all laboratory exercises work, and scientists need a way to check to see if their transgenic bacteria picked up the donor gene. • To test this, an antibiotic resistant gene is often also transferred with the desired donor gene. • We can then grow the bacteria up on a plate containing the antibiotic in the agar. Only resistant bacteria survive, and these will also contain the donor gene that we’re looking for.

10. Other Uses of Restriction Enzymes • Digesting DNA fragments for Fingerprinting. • DNA is digested, loaded into a gel, and you end up with bands of DNA • Bands are characteristic of each person, and can be used as a “Fingerprint” (also known as RFLP= Restriction Fragment Length Polymorphism)

11. Uses of DNA Technology • Gene Therapy-fixing genes with mistakes in an adult organism • PCR-Copying mass quantities of DNA fragments • Cloning-using nuclear switches of stem cells to create identical organisms • Human Genome Project (HGP)-Sequencing and Mapping all of the genes in the human genome.

12. Test Topics • Steps to creating transgenic organisms • Purpose of each step • Steps of cloning • Uses of stem cells • Pros/Cons of human gene therapy • Steps to RFLP analysis • Uses for RFLP