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Myr-Akt1

Additional figure S1. The myr-Akt1 transgene and integration of the transgene in transgenic mice. 0.01 copies. Map of transgene. A. 25. Linearized transgene fragment. B. 20. Threshold Cycle. 15. Poly A tail. Myr-Akt1-myc-tag. MMTV-LTR. 10. 2.5. Myr-Akt1. 5. Myc-His. R 2 =0.919.

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Myr-Akt1

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  1. Additional figure S1 The myr-Akt1 transgene and integration of the transgene in transgenic mice 0.01 copies Map of transgene A 25 Linearized transgene fragment B 20 Threshold Cycle 15 Poly A tail Myr-Akt1-myc-tag MMTV-LTR 10 2.5 Myr-Akt1 5 Myc-His R2 =0.919 100 copies 0 2 -3 -2 -1 0 1 2 3 Log Starting Quantity, Copy number MMTV-LTR 1.5 Gene Copy Number SV40 ori D. Estimate copy numbers in transgenic mice pUSEamp 1 C Copy number standard curve 0.5 Neo Amp 0 2-14 2-10 2-11 2-12 2-33 2-21 2-23 SV40 PA Transgenic Mice

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