1 / 2

Mock

Supplementary Fig 1. Mock. NDRG2. A. **. 1.25. Integrin β 6. 1.00. Fold induction. 0.75. PAI-1. 0.50. NDRG2. 0.25. GAPDH. 0.00. Mock. NDRG2. B. Real time PCR.

satin
Download Presentation

Mock

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplementary Fig 1 Mock NDRG2 A ** 1.25 Integrin β6 1.00 Fold induction 0.75 PAI-1 0.50 NDRG2 0.25 GAPDH 0.00 Mock NDRG2 B Real time PCR Supplementary Fig 1. NDRG2 attenuated TGF--mediated signaling pathway. (A)Effect of NDRG2 on transcriptional expression of integrin 6 and PAI-1 which are associated with TGF--mediated signaling. (B) The spontaneous SBE activation of 4T1-Mock and 4T1-NDRG2 cells which were transfected with SBE-luciferase construct. **P < 0.01, by t-test.

  2. Supplementary Fig 2 NDRG2 NDRG2 Mock Mock Integrin 6 PAI-1 NDRG2 20 15 15 ** TGF-β1 TGF-β R1 ** 10 10 TGF-β2 TGF-β R2 Relative expression 10 5 5 TGF-β3 TGF-β R3 0 0 0 GAPDH GAPDH M M M N N+T N N+T N N+T B A Supplementary Fig 2. Comparison of gene expression related with TGF- signaling in 4T1-mock and 4T1-NDRG2. (A)mRNA levels of TGF- subtypes and their receptors in 4T1-mock and 4T1-NDRG2 cells. (B) mRNA levels of TGF- target genes, integrin6 and PAI-1, in 4T1-mock and 4T1-NDRG2 treated with recombinant TGF-. M: 4T1-Mock; N: 4T1-NDRG2; N+T: 4T1-NDRG2 treated with TGF-. **P < 0.01, by t-test.

More Related