80 likes | 183 Views
Chromosome 1 addition line. Unusual characteristics noticed in chromosome 1 OMA lines:Erect leaf blade, photoperiod neutral response, sectoring among shoots for the maize chromosome. Chromosome 10 Addition Line. Characteristics of chromosome 10 OMA lines: grassy type and very sterile.
E N D
Chromosome 1 addition line Unusual characteristics noticed in chromosome 1 OMA lines:Erect leaf blade, photoperiod neutral response, sectoring among shoots for the maize chromosome
Chromosome 10 Addition Line Characteristics of chromosome 10 OMA lines: grassy type and very sterile
Oat x maize addition line Genomic in situ hybridization of an addition line with fluorescein-labeled maize DNA. The pair of maize chromosomes are yellow-green, and the oat chromosomes are counterstained red with propidium iodide.
30-40 krad γ-rays Oat-corn chromosome 9 monosomic addition line Selfing Oat line without a corn chromosome Oat line with a corn chromosome piece transfer Oat line with a modified corn chromosome
Mapping with OMA lines primer33C primer33A primer34A primer34C primer34A: will amplify ESTs AW066336 and AW066011. These two sequences are very similar, e-172 and 335/343 (97%) and may represent two alleles from one gene. primer34C: was designed to amplify TC77059. This sequence is more distantly related to AW066336, e-112, 269/289 (93%). The gene represented by ESTs AW066336 and AW066011 appears to lie on chromosome 5. The PCR primers, primer34C, detects the related sequence on chromosome 4.