Karl Reich, Ph.D. Independent Forensics Lombard IL

1. A New and Better Forensic Test for Human Blood Karl Reich, Ph.D. Independent Forensics Lombard IL

2. extract in provided extraction buffer adsorb stain onto swab 15 min add to RSID-Blood mix extract aliquot with running buffer (100 ml total) RSIDTM Running Buffer BLOOD

3. extract in provided extraction buffer adsorb stain onto swab 15 min add to RSID-Blood mix extract aliquot with running buffer (100 ml total) RSIDTM Running Buffer BLOOD

4. Extract volume Equiv. vol human blood 20 ml 0 ml 1 ml@1:5 1 ml 5 ml 100 ml 0 ml 10 nl 50 nl 250 nl 1 ml 5 ml BLOOD Sensitivity Testing 50 ml of human blood extracted in 1 ml BEB

5. Total Extract Volume (ml) 1,000 0 500 200 Equiv. volume human blood (ml) 0 5 10 25 BLOOD No High Dose Hook Effect

6. neg pos F C G BLOOD TestingRSID-BLOODTMfor Species Specificity 20 ml of Extract 1 ml equivalent neg – negative control pos – positive control F –ferret C –bonobo chimpanzee G – gorilla

7. RSID-BLOODTM 1 ml 20 ml 1 ml 20 ml 1 ml 20 ml neg pos Seratec Hemedirect ferret gorilla orangutan neg H F G O Hexagon OBTI BLOOD Species Specificity: Direct Comparison

8. BLOOD

9. BLOOD Specificity Testing Animal Species Tested: ferret, orangutan, gorilla, lowland gorilla, baboon, spider monkey, skunk, bonobo chimpanzee, goat, sheep, pig, horse, cat, mouse, dog, bull, Box turtle, opossum, American Elk, mule deer, alpaca, Siberian tiger, Nubian goat, Body Fluids Tested: sweat, semen, urine, saliva, vaginal secretions, double mixtures, triple mixtures NO CROSS REACTIONS IDENTIFIED - INCLUDING PRIMATES – CONFIRMATORY FOR HUMAN BLOOD

10. Comments Samples Gripes Questions Karl Reich Tel 708.234.1200 Fax 708.978.5115 karl@IFI-test.com www.IFI-test.com