Anovel approach for the spectrophotometric determination of tryptophan in drug samples of different origins using homema
Anew, rapid and sensitive batch and flow injection u00f1 merging zones spectrophotometric methods for the determination of Tryptophan in pure material and pharmaceutical preparations were proposed. These methods based on oxidation of the reagent Diphenyl amine sulphonate (DASA) to diphe- nyl benzidine sulphonic acid after reaction with sodium nitrite in the sulphuric acid medium. The unstable oxidation product react quickly with sodium nitrite to produce a diazotized intermediate. The diazotized interme- diate is coupled with Trp., a pink color product was developed which is stable for 1 h in 30 0C. The colored product measured at 522nm.
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