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Method development and validation for simultaneous estimation of Paracetamol and Etoricoxib in pharmaceutical dosage for

Method development and validation for simultaneous estimation of Paracetamol and Etoricoxib in pharmaceutical dosage for

A RP-HPLC method was developed for simultaneous estimation of Paracetamol and Etoricoxib tablet formulation using Phenomenex Luna C18 column (250 mm × 4.6 mm id, 5 µm particle size) and a mobile phase of methanol : water (70:30 v/v), at flow rate 1.0 ml/min with UV detection at 235 nm. The retention time (tR) of Paracetamol and Etoricoxib found to be 3.07 and 5.72 min respectively. The proposed method was validated for system suitability, specificity, linearity, accuracy, precision, LOD, LOQ and robustness. All parameters were found to be within the acceptance limit. Linearity over the concentration range 5-30 µg/ml for both Paracetamol and Etoricoxib with regression coefficient (r2 ) 0.9998 and 0.9994 respectively. Limit of detection (LOD) found to be 0.10 µg/ml and 0.04 µg/ml whereas limit of quantitation (LOQ) found to be 0.33 µg/ml and 0.13 µg/ml for Paracetamol and Etoricoxib respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.74%- 101.25% and 99.08%-99.21% for Paracetamol and Etoricoxib respectively.

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Analytical Method Development and Validation of Teneligliptin by using RP HPLC with ICH Guidelines

Analytical Method Development and Validation of Teneligliptin by using RP HPLC with ICH Guidelines

Teneligliptin is drug used against type 2 diabetes mellitus and it is also a member of class of anti diabetic drugs known as dipeptidyl peptidase 4 inhibitors or gliptins . A simple, sensitive and accurate RP HPLC method has been developed for the determination of Teneligliptin in bulk formulation. The max of the Teneligliptin was found to be 246 nm in Methanol Phosphate buffer pH 3 70 30 v v . The method shows high sensitivity with linearity 10 to 50 u00b5-g ml regression equation y = 54647x 74133 r2 = 0.9968 . The various parameters according to ICH guidelines are followed for validating and testing of this method. The Detection limit and quantitation limit were found to be 0.109 u00b5-g ml 1 and 0.3305 u00b5-g ml1 in Methanol Phosphate buffer pH 3 70 30 v v respectively. The purity of tablet formulation was found to be 99.57 . The results demonstrated that the procedure is accurate, specific and reproducible RSD 2 , and also being simple, cheap and less time consuming and appropriate for the determination of Teneligliptin in bulk and pharmaceutical formulation. Dr. Pradnya Lokhande "Analytical Method Development and Validation of Teneligliptin by using RP-HPLC with ICH Guidelines" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21735.pdf Paper URL: https://www.ijtsrd.com/chemistry/chromatography/21735/analytical-method-development-and-validation-of-teneligliptin-by-using-rp-hplc-with-ich-guidelines/dr-pradnya-lokhande

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