'Cdna libraries' presentation slideshows

658 views • 39 slides

Screening, selection & characterisation

Screening, selection & characterisation Survey of cDNA cloning strategies/tools/procedures/interconnections Plating out a library : cfu/ml or pfu/ml (suspension) low density (individual clones) or high density (up to confluent lawn)

700 views • 38 slides

PBIO 427/527: Molecular Genetics Lecture 2 - Review

PBIO 427/527: Molecular Genetics Lecture 2 - Review. Prokaryotic gene structure, processing & regulation Eukaryotic gene structure, processing & regulation Restriction enzymes & gel electrophoresis DNA cloning & cloning vectors Gene libraries & screening cDNA libraries & screening.

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DNA Sequence Analysis

DNA Sequence Analysis. 5.1 Introduction 1. Terms in common use are defined, and the genetic code is reviewed. 2. EST-Expressed Sequence Tag as a unit of sequence data, derived from rapid sequencing of cDNA libraries. 3. Three examples of producers of EST databases are profiled.

1.21k views • 30 slides

The Laccaria transcriptome: ESTs and Microarrays

The Laccaria transcriptome: ESTs and Microarrays. EST resources Nylon array NimbleGen genome expression array Symbion Chip (JGI). cDNA libraries. CAHB, CAHC, CAHF,CAHG, cDNA libraries from mixed tissues (JGI) CAHH, CAPI,CAPN (mainly mycelium) 30826 ESTs LU ? 165 ESTs

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LECTURES 3/4. CONSTRUCTING and

LECTURES 3/4. CONSTRUCTING and. SCREENING cDNA LIBRARIES to. ISOLATE NEW GENES. ORIGINAL ARTICLES:. CLONING BY COMPLEMENTATION: Lew, D, Dulic, V, and Reed SI. 1991. Isolation of three. novel human cyclins by rescue of G1 Cyclin (Cln) in yeast. Cell 66:1127-1206.

311 views • 23 slides

Current Laccaria transcriptomics ressources: cDNA libraries, ESTs, cDNA arrays

Current Laccaria transcriptomics ressources: cDNA libraries, ESTs, cDNA arrays. A Kohler, M Peter, F Duchaussoy, A Deveau, C Delaruelle P Brokstein, E Lindquist, PM Richardson et F Martin. I. cDNA libraries. CAHB, CAHC, CAHF,CAHG, cDNA libraries from mixed tissues (JGI)

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Biotechnology and Genetic Engineering PBIO 4500/5500

Biotechnology and Genetic Engineering PBIO 4500/5500. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

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Genomic DNA & cDNA Libraries

Genomic DNA & cDNA Libraries. By: Tal H Hasson. Introduction. The use of genetic information is a powerful tool that today is becoming more readily available to scientists.

838 views • 15 slides

Introduction

Introduction

100 views • 1 slides