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Genome Analysis

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Species Richness in Soil Bacterial Communities: A Proposed Approach to Overcome Sample-Size Bias

Methods : DNA extraction from 0.5 g of soil using a modified lysis and bead-beating protocol. PCR : A near complete 16S rRNA gene fragment was amplified using primer pair 27F and 1391R. Cloning : Invitrogen TOPO-TA cloning kit.

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Clone library - PowerPoint PPT Presentation

Genome Analysis

Species Richness in Soil Bacterial Communities: A Proposed Approach to Overcome Sample-Size Bias