'Colony pcr' presentation slideshows

In Vivo assay for RNA-protein interactions

In Vivo assay for RNA-protein interactions. Dana M. Schneider Loren Williams lab. Outline. The Ribosome Yeast three hybrid assay for RNA-protein interactions Construct vectors PCR amplify insert Restriction digest plasmids and insert Ligate vector and insert

885 views • 25 slides

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics Complementarity of NMR and Xray Crystallography. Protein Production Technologies for NESG Consortium. E. coli Production Vectors pET based with HexaHis Tags - multiplex vector set

341 views • 25 slides

Cloning of the genes that code for three major subunits of Escherichia coli polymerase III

Cloning of the genes that code for three major subunits of Escherichia coli polymerase III. Chengxi Shi Molecular Biotechnology and Bioinformatics Uppsala University winter,2005. About Research Training. Period: November to December

405 views • 24 slides

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics Complementarity of NMR and Xray Crystallography. Protein Production Technologies for NESG Consortium. E. coli Production Vectors pET based with HexaHis Tags - multiplex vector set

300 views • 17 slides

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics

Eukaryotic Gene Families Coverage of Structure Space Prioritization by Functional Genomics Complementarity of NMR and Xray Crystallography. Protein Production Technologies for NESG Consortium. E. coli Production Vectors pET based with HexaHis Tags - multiplex vector set

254 views • 17 slides

Academic Report

Academic Report. Zhongdi Song Date:2012-10-20. Contents. Gene K onckout. Colony PCR. Phenotypic Screening. Gene konckout. Eight genes sll1734, sll1549, sll0258, sll0057 slr1339 , slr1472, ssr1853,ssr3402 Basic operation primer design

364 views • 13 slides

& SPECIAL TECHNIQUES

270 views • 12 slides

BP Reaction

BP Reaction. LR Reaction. Gateway cloning system. Why Gateway. No restriction enzymes needed Fast High efficiency Multiple destination vectors Site-directed mutagenesis (Empty donor vector is very small ~2.3kb). 1. Design the primers. attB1(Foward): GGGGACAAGTTTGTACAAAAAAGCAGGCT TC

598 views • 11 slides