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Protein Crosslinking

Crosslinking is a technique that covalently binds two or more biomolecules together to form conjugates with enhanced physical properties and/or biological functionalities. The structure of a functioning protein involves more than just the intramolecular covalent bonds, which results in challenging questions regarding the spatial orientation and non-covalent interactions of the protein. The dynamic nature of proteins in physiological environment makes it even difficult to determine the roles of individual amino acids in the chemical and biological properties of proteins.

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