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Double stranded pcr product - PowerPoint PPT Presentation

Strategies and Results of Routine Semi-automated Cloning

Strategies and Results of Routine Semi-automated Cloning. Heath Klock - JCSG. Our Vector Strategy. Small, non-cleavable expression/purification tag directly followed by the gene of interest Blunt/Pac strategy directs orientation while eliminating extra codons/residues in the N-terminal tag

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Double stranded pcr product - PowerPoint PPT Presentation

Strategies and Results of Routine Semi-automated Cloning