100+ Enzymatic removal PowerPoint (PPT) Presentations, Enzymatic removal PPTs

Enzymatic removal - PowerPoint PPT Presentation

SDS-PAGE and Western Analysis

SDS-PAGE and Western Analysis. SDS-PAGE purposes. To separate protein molecules on the basis of molecular weight and To determine the molecular weights of unknowns by comparison to standards. SDS-PAGE preparation of cell extract.

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975 views • 47 slides

SDS-PAGE

SDS-PAGE. SDS-PAGE purposes. To separate protein molecules on the basis of molecular weight May then be electroblotted for immunoanalysis To determine the molecular weights of unknowns by comparison to standards. HRP. + ECL substrate ? light. HRP conjugated 2 o antibody. 1 o antibody.

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1.48k views • 32 slides

Protein modification

Protein modification. 16-1. Protein modifications different types, including co-translational and post-translational inteins. It is estimated that the human proteome consists of ~300,000 different proteins, or about 10X more than the number of genes (!)

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804 views • 15 slides

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Enzymatic removal - PowerPoint PPT Presentation

SDS-PAGE and Western Analysis

SDS-PAGE

Protein modification