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Immunofluorescence and Confocal Microscopy

Immunofluorescence and Confocal Microscopy. Dr. KW Chan. Part I: Immunofluorescence. Learning Objectives. To understand the working principles of immunofluorescence microscope To understand the difference between direct and indirect immunofluorescence

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Immunofluorescence Microscopy

Immunofluorescence Microscopy. Immunofluorescence Microscopy:. When an antibody, or the antiimmunoglobulin antibody used to detect the antibody is labeled with a fluorescent dye This method is used when looking at the subcellular location of a protein of interest.

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Fluorescent labels - PowerPoint PPT Presentation

Immunofluorescence and Confocal Microscopy

Immunofluorescence Microscopy