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Formula 2 dd ct - PowerPoint PPT Presentation

Sample (dissected frozen brain region)

Data generation. Sample (dissected frozen brain region). RNA extraction (total RNA) QIAZOL and RNeasy mini kit. RNA quantification (Bioanalyser). Reverse transcription MMLV RT-ase Random priming. Q-PCR with gene-specific primers Multiple reference genes Genes of interest.

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Formula 2 dd ct - PowerPoint PPT Presentation

Sample (dissected frozen brain region)