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HPLC reduced ASIP after Nickel Column

The Agouti Signaling Protein (ASIP) Darren A. Thompson and Glenn L. Millhauser University of California at Santa Cruz. Absorbance 214 nm Absorbance 280 nm. appears as banded gray. Grow BL21 in LB Miller broth Induce with IPTG Lyse cells Affinity purify Ni-NTA.

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HPLC reduced ASIP after Nickel Column