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A) Genomic Section of DNA 19.8 Kb cloned in 3 Kb vector, therefore total size of pSP1 = 22.8 Kb

A) Genomic Section of DNA 19.8 Kb cloned in 3 Kb vector, therefore total size of pSP1 = 22.8 Kb Fully digested with Hind III gives 9.7, 7.5, 2.8, 2.3 and 0.5 (sum of fragments = 22.8 Kb) therefore no double bands are present.

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A) Genomic Section of DNA 19.8 Kb cloned in 3 Kb vector, therefore total size of pSP1 = 22.8 Kb