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Validated TLC-Densitometric Method for Determination of Amprolium Hydrochloride and Ethopabate in Veterinary Preparation

A simple precise accurate TLC- densitometric method was presented for the quantitative determination of amprolium hydrochloride and ethopabate in combined veterinary preparations and in presence of Levamisole hydrochloride. The compounds were developed on TLC aluminum plates pre-coated with silica gel 60 F254 using methanol: water: 0.1% acetic acid (7:2.5:0.5, v/v/v). The plates were air dried, detected under UV lamp and then scanned at 213 nm. The validity of the proposed method was assessed using the standard addition technique. The obtained results were statistically compared with those obtained by the official method, showing no significant difference with respect to accuracy and precision at p=0.05.

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